Journal: Endocrinology

Article Title: Glucose Transporter-4 Facilitates Insulin-Stimulated Glucose Uptake in Osteoblasts

doi: 10.1210/en.2016-1583

Figure Lengend Snippet: Antibody Table

Article Snippet: /article/back/app-group/app/ See . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Peptide/Protein Target Antigen Sequence (if Known) Name of Antibody Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody Species Raised in; Monoclonal or Polyclonal Dilution Used Antibody Registry ID Glut4 Glut4 (1F8) Cell Signaling Technologies, 2213 Mouse; monoclonal 1:1000 AB_823508 IR IR-β (4B8) Cell Signaling Technologies, 3025 Rabbit; monoclonal 1:2000 AB_2280448 IGF-1 receptor IGF-1Rβ (111A9) Cell Signaling Technologies, 3018 Rabbit; monoclonal 1:2000 AB_10693453 Actin β-Actin (8H10D10) Cell Signaling Technologies, 3700 Mouse; monoclonal 1:10 000 AB_2242334 Insulin Insulin DAKO, A056401-2 Guinea pig; polyclonal 1:1000 AB_2617169 Open in a separate window Antibody Table

Techniques: Sequencing

Journal: ImmunoHorizons

Article Title: Serendipitous Discovery of T Cell–Produced KLK1b22 as a Regulator of Systemic Metabolism

doi: 10.4049/immunohorizons.2300016

Figure Lengend Snippet: T-Rheb −/− mice are heavier than control mice but demonstrate improved glucose and insulin tolerance. ( A ) Summary data of mouse weight on a normal chow diet starting at 6 wk of age. ( B ) Mice were fasted overnight; serum was isolated; and total cholesterol, triglycerides, and HDL were interrogated. ( C ) Mice were fasted for 7 h, and fasting blood glucose levels were obtained. ( D ) Mice were fasted for 7 h, then challenged with 1 g/kg glucose i.p., and blood glucose was measured over the indicated time course. ( E ) Mice were fasted for 2 h, then challenged with 0.5 U/kg insulin i.p., and blood glucose was measured over the indicated time course. ( F ) Left: Inguinal WAT (WAT Ing) was interrogated for phospho-IGF-I receptor β (P-IGF-I R β) (Y1131)/phospho-insulin receptor β (P-IR β) (Y1146) signaling via Western blot analysis. Right: The ratio of p-IR and total IR was determined by densitometry. ( G ) Western blot analysis performed on inguinal WAT from WT and T-Rheb −/− mice. ( H ) Western blot analysis performed on pectoralis major skeletal muscle from WT and T-Rheb −/− mice. Data are representative of three or more independent experiments of 5-mo-old mice (A, C–E) or 3-mo-old mice (B, F–H). An unpaired t test was performed for (B), (C), and (F), and repeated measures two-way ANOVA was performed for (A), (D), and (E) for statistical analysis (* p < 0.05, ** p ≤ 0.01, **** p ≤ 0.0001). n = 4–10 mice per group. Data are mean ± SEM.

Article Snippet: The following Abs were purchased from Cell Signaling Technology: anti-succinate dehydrogenase (SDHA) (D6J9M, 11998), anti-voltage-dependent anion channel (VDAC) (D73D12, 4661), anti-phospho-IGF-I receptor β (Tyr1131)/insulin receptor β (Tyr1146) (3021), anti-insulin receptor β (4B8, 3025), glucose transporter 4 (Glut4) (1F8, 2213), and anti-β-actin (D6A8, 8457).

Techniques: Isolation, Western Blot

Journal: Endocrinology

Article Title: Roles of Growth Hormone–Dependent JAK-STAT5 and Lyn Kinase Signaling in Determining Lifespan and Cancer Incidence

doi: 10.1210/endocr/bqae136

Figure Lengend Snippet: Antibodies used in this study

Article Snippet: INSRβ (4B8) , 3025 , AB 2280448 , Cell Signaling Technology.

Techniques: